PrimePCR™ SYBR® Green Assay: Gm7945, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: Gm7945, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Gm7945, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020976
Assay Design:   Intron-spanning
Chromosome Location:   14:41385157-41386376question
Amplicon Length:   194
Splice Variants Targeted:   ENSMUST00000170249 ENSMUST00000164948 ENSMUST00000169361 ENSMUST00000111869 ENSMUST00000111870 ENSMUST00000100695 ENSMUST00000169756 ENSMUST00000178178 ENSMUST00000163970 ENSMUST00000096899 ENSMUST00000107289 ENSMUST00000174180 ENSMUST00000175955 ENSMUST00000051605 ENSMUST00000019143 ENSMUST00000039059

Gene Information

Description Not Available

Gene Symbol:   Gm7945
Gene Name:   Predicted gene 7945
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000091131
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.500000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download