PrimePCR™ SYBR® Green Assay: Marcks, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020960
Assay Design:   Intron-spanning
Chromosome Location:   10:37136856-37138580question
Amplicon Length:   197
Splice Variants Targeted:   ENSMUST00000086210 ENSMUST00000172743 ENSMUST00000167771 ENSMUST00000098006 ENSMUST00000084474 ENSMUST00000052107 ENSMUST00000091851 ENSMUST00000109896 ENSMUST00000030014 ENSMUST00000027455 ENSMUST00000041537 ENSMUST00000021028 ENSMUST00000166232 ENSMUST00000092995 ENSMUST00000030826 ENSMUST00000170580 ENSMUST00000092584 ENSMUST00000006559 ENSMUST00000130451 ENSMUST00000052590 ENSMUST00000064709 ENSMUST00000119254 ENSMUST00000098614 ENSMUST00000137724 ENSMUST00000030879 ENSMUST00000161908 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Marcks
Gene Name:   Myristoylated alanine rich protein kinase C substrate
Aliases:   Macs, PKCSL
RefSeq:   NC_000076.6 NT_039492.8
Ensembl:   ENSMUSG00000069662
Entrez:   17118
UniGene:   Mm.30059
Chromosome Mapping:   10 B1|10 22.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.480000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download