PrimePCR™ SYBR® Green Assay: 5430421N21Rik, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: 5430421N21Rik, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: 5430421N21Rik, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020958
Assay Design:   Intron-spanning
Chromosome Location:   15:101488403-101489654question
Amplicon Length:   71
Splice Variants Targeted:   ENSMUST00000106969 ENSMUST00000106970 ENSMUST00000081945 ENSMUST00000023718 ENSMUST00000170373 ENSMUST00000025668 ENSMUST00000113717 ENSMUST00000100225 ENSMUST00000095083 ENSMUST00000046257 ENSMUST00000113719 ENSMUST00000020732 ENSMUST00000133040 ENSMUST00000047441 ENSMUST00000081658 ENSMUST00000052872 ENSMUST00000047005 ENSMUST00000108305 ENSMUST00000109558

Gene Information

Description Not Available

Gene Symbol:   5430421N21Rik
Gene Name:   RIKEN cDNA 5430421N21 gene
Aliases:   Not Available
RefSeq:   NC_000081.6 NT_039621.8
Ensembl:   ENSMUSG00000067613
Entrez:   100126226
Chromosome Mapping:   15|15

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 35.700000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download