PrimePCR™ SYBR® Green Assay: Cyp2a12, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020952
Assay Design:   Intron-spanning
Chromosome Location:   7:27035311-27036515question
Amplicon Length:   163
Splice Variants Targeted:   ENSMUST00000023812 ENSMUST00000171565 ENSMUST00000169377 ENSMUST00000021183 ENSMUST00000170227 ENSMUST00000075552 ENSMUST00000019232 ENSMUST00000075821 ENSMUST00000119361

Gene Information

Description Not Available

Gene Symbol:   Cyp2a12
Gene Name:   Cytochrome P450, family 2, subfamily a, polypeptide 12
Aliases:   Not Available
RefSeq:   NC_000073.6 NT_187034.1
Ensembl:   ENSMUSG00000060407
Entrez:   13085
UniGene:   Mm.20770
Chromosome Mapping:   7 A3|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.980000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download