PrimePCR™ SYBR® Green Assay: FP885536.1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: FP885536.1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FP885536.1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020947
Assay Design:   Intron-spanning
Chromosome Location:   4:156336904-156338237question
Amplicon Length:   182
Splice Variants Targeted:   ENSMUST00000159674 ENSMUST00000071277 ENSMUST00000178970 ENSMUST00000165180 ENSMUST00000031651 ENSMUST00000117488 ENSMUST00000038379 ENSMUST00000100167 ENSMUST00000050029 ENSMUST00000161976 ENSMUST00000022971 ENSMUST00000115154 ENSMUST00000075406 ENSMUST00000079949 ENSMUST00000114812 ENSMUST00000039855 ENSMUST00000096792

Gene Information

Description Not Available

Gene Symbol:   FP885536.1
Gene Name:   Vomeronasal 2, receptor 123 precursor
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000057021
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.997000
y-intercept 35.840000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download