PrimePCR™ SYBR® Green Assay: Fbxl22, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020941
Assay Design:   Intron-spanning
Chromosome Location:   9:66511911-66514265question
Amplicon Length:   125
Splice Variants Targeted:   ENSMUST00000150285 ENSMUST00000036195 ENSMUST00000052740 ENSMUST00000025234 ENSMUST00000052107 ENSMUST00000035836 ENSMUST00000165052 ENSMUST00000106507 ENSMUST00000032892 ENSMUST00000093907 ENSMUST00000106354 ENSMUST00000019038 ENSMUST00000100193 ENSMUST00000106349 ENSMUST00000102946 ENSMUST00000021785 ENSMUST00000117095 ENSMUST00000117611 ENSMUST00000044338 ENSMUST00000145322 ENSMUST00000081857 ENSMUST00000113130 ENSMUST00000061013 ENSMUST00000105031 ENSMUST00000106213 ENSMUST00000134458 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Fbxl22
Gene Name:   F-box and leucine-rich repeat protein 22
Aliases:   1110004B15Rik
RefSeq:   NC_000075.6 NT_039474.8
Ensembl:   ENSMUSG00000050503
Entrez:   74165
UniGene:   Mm.297998
Chromosome Mapping:   9 C|9

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.400000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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