PrimePCR™ SYBR® Green Assay: Klhl11, Mouse

print
PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020938
Assay Design:   Intron-spanning
Chromosome Location:   11:100464352-100472315question
Amplicon Length:   199
Splice Variants Targeted:   ENSMUST00000153983 ENSMUST00000106539 ENSMUST00000056946 ENSMUST00000056665 ENSMUST00000098660 ENSMUST00000171654 ENSMUST00000044053 ENSMUST00000054814 ENSMUST00000159541 ENSMUST00000178075 ENSMUST00000085044 ENSMUST00000006854 ENSMUST00000030133 ENSMUST00000136872 ENSMUST00000028820

Gene Information

Description Not Available

Gene Symbol:   Klhl11
Gene Name:   Kelch-like 11 (Drosophila)
Aliases:   BC011167, RP23-392I3.12
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000048732
Entrez:   217194
UniGene:   Mm.183320
Chromosome Mapping:   11 D|11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.440000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
Get Help

Call us at 1-800-4-BIORAD (1-800-424-6723)

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.