PrimePCR™ SYBR® Green Assay: Tob2, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020937
Assay Design:   Intron-spanning
Chromosome Location:   15:81851841-81858065question
Amplicon Length:   132
Splice Variants Targeted:   ENSMUST00000026156 ENSMUST00000094870 ENSMUST00000076949 ENSMUST00000025264 ENSMUST00000112149 ENSMUST00000174320 ENSMUST00000179032 ENSMUST00000110611 ENSMUST00000036715 ENSMUST00000077133 ENSMUST00000110596 ENSMUST00000179214 ENSMUST00000110595 ENSMUST00000148078 ENSMUST00000141250 ENSMUST00000035548 ENSMUST00000047025 ENSMUST00000057407 ENSMUST00000036453 ENSMUST00000108341 ENSMUST00000032918 ENSMUST00000037165 ENSMUST00000050467 ENSMUST00000107737 ENSMUST00000107738 ENSMUST00000095176 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Tob2
Gene Name:   Transducer of ERBB2, 2
Aliases:   2900090N22Rik, 4930545K18Rik, AV071822, mKIAA1663
RefSeq:   NC_000081.6 NT_039621.8
Ensembl:   ENSMUSG00000048546
Entrez:   57259
UniGene:   Mm.323595
Chromosome Mapping:   15|15 E2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.510000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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