PrimePCR™ SYBR® Green Assay: Rtp2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020935
Assay Design:   Intron-spanning
Chromosome Location:   16:23927639-23930678question
Amplicon Length:   191
Splice Variants Targeted:   ENSMUST00000055655 ENSMUST00000163770 ENSMUST00000043189 ENSMUST00000103091 ENSMUST00000074046 ENSMUST00000094329 ENSMUST00000103088 ENSMUST00000071699 ENSMUST00000106429 ENSMUST00000145529 ENSMUST00000034190 ENSMUST00000026381 ENSMUST00000164016 ENSMUST00000090559 ENSMUST00000111278 ENSMUST00000056567 ENSMUST00000081393 ENSMUST00000038730 ENSMUST00000061030 ENSMUST00000171796 ENSMUST00000171556 ENSMUST00000112958 ENSMUST00000086819 ENSMUST00000001080 ENSMUST00000027494 ENSMUST00000175866 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Rtp2
Gene Name:   Receptor transporter protein 2
Aliases:   Gm605
RefSeq:   NC_000082.6 NT_039624.8
Ensembl:   ENSMUSG00000047531
Entrez:   224055
UniGene:   Mm.39248
Chromosome Mapping:   16 B1|16

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.700000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download