PrimePCR™ SYBR® Green Assay: Shisa2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: Shisa2, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Shisa2, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020922
Assay Design:   Intron-spanning
Chromosome Location:   14:59626368-59629710question
Amplicon Length:   79
Splice Variants Targeted:   ENSMUST00000164826 ENSMUST00000160424 ENSMUST00000056028 ENSMUST00000026662 ENSMUST00000106231 ENSMUST00000022099 ENSMUST00000044442 ENSMUST00000160964 ENSMUST00000031077 ENSMUST00000091237 ENSMUST00000105852 ENSMUST00000027502 ENSMUST00000057090 ENSMUST00000117386 ENSMUST00000119245 ENSMUST00000179395 ENSMUST00000053949 ENSMUST00000121964

Gene Information

Description Not Available

Gene Symbol:   Shisa2
Gene Name:   Shisa homolog 2 (Xenopus laevis)
Aliases:   9430059P22Rik, MAd2, Tmem46, mShisa, shisa
RefSeq:   NC_000080.6 NT_039606.8
Ensembl:   ENSMUSG00000044461
Entrez:   219134
Chromosome Mapping:   14 D1|14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.620000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download