PrimePCR™ SYBR® Green Assay: Onecut1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020919
Assay Design:   Intron-spanning
Chromosome Location:   9:74863279-74889362question
Amplicon Length:   150
Splice Variants Targeted:   ENSMUST00000025486 ENSMUST00000084696 ENSMUST00000056006 ENSMUST00000078209 ENSMUST00000153965 ENSMUST00000035121 ENSMUST00000130602 ENSMUST00000034862 ENSMUST00000127906 ENSMUST00000124830 ENSMUST00000119499 ENSMUST00000121409 ENSMUST00000020411 ENSMUST00000179218 ENSMUST00000021609 ENSMUST00000039537 ENSMUST00000107187 ENSMUST00000168223 ENSMUST00000098761 ENSMUST00000154460 ENSMUST00000105307 ENSMUST00000020231 ENSMUST00000031280 ENSMUST00000128302 ENSMUST00000115526 ENSMUST00000026045 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Onecut1
Gene Name:   One cut domain, family member 1
Aliases:   D9Ertd423e, HNF-6, Hfh12, Hnf6, Hnf6b, OC-1, Oc1
RefSeq:   NC_000075.6 NT_039474.8
Ensembl:   ENSMUSG00000043013
Entrez:   15379
UniGene:   Mm.303355
Chromosome Mapping:   9 D|9 42.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.840000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download