PrimePCR™ SYBR® Green Assay: Spred3, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: Spred3, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Spred3, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020903
Assay Design:   Intron-spanning
Chromosome Location:   7:29162958-29166631question
Amplicon Length:   188
Splice Variants Targeted:   ENSMUST00000076734 ENSMUST00000087877 ENSMUST00000021173 ENSMUST00000106296 ENSMUST00000144153 ENSMUST00000092382 ENSMUST00000048923 ENSMUST00000006470 ENSMUST00000108154 ENSMUST00000017090 ENSMUST00000137454 ENSMUST00000119373 ENSMUST00000165104 ENSMUST00000073192 ENSMUST00000107464 ENSMUST00000090924 ENSMUST00000041022 ENSMUST00000102521 ENSMUST00000047488 ENSMUST00000048371 ENSMUST00000034281 ENSMUST00000108951 ENSMUST00000108950 ENSMUST00000038907

Gene Information

Description Not Available

Gene Symbol:   Spred3
Gene Name:   Sprouty-related, EVH1 domain containing 3
Aliases:   AW047143, D130060H24Rik, Spred-3
RefSeq:   NC_000073.6 NT_187034.1
Ensembl:   ENSMUSG00000037239
Entrez:   101809
Chromosome Mapping:   7 B1|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.560000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download