PrimePCR™ SYBR® Green Assay: Nhlh2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020874
Assay Design:   Intron-spanning
Chromosome Location:   3:102011022-102012795question
Amplicon Length:   158
Splice Variants Targeted:   ENSMUST00000031894 ENSMUST00000163936 ENSMUST00000114684 ENSMUST00000024967 ENSMUST00000066187 ENSMUST00000010451

Gene Information

Description Not Available

Gene Symbol:   Nhlh2
Gene Name:   Nescient helix loop helix 2
Aliases:   6230401I09Rik, Hen2, NSCL2, Nscl-2, bHLHa34
RefSeq:   NC_000069.6 NT_039240.8
Ensembl:   ENSMUSG00000048540
Entrez:   18072
UniGene:   Mm.137286
Chromosome Mapping:   3 F2.2|3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.010000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download