PrimePCR™ SYBR® Green Assay: Nkx3-2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020872
Assay Design:   Intron-spanning
Chromosome Location:   5:41762118-41763516question
Amplicon Length:   68
Splice Variants Targeted:   ENSMUST00000001712 ENSMUST00000031099 ENSMUST00000001975 ENSMUST00000081857 ENSMUST00000079178 ENSMUST00000115224 ENSMUST00000060820 ENSMUST00000027559 ENSMUST00000108987 ENSMUST00000117061 ENSMUST00000142911 ENSMUST00000148287 ENSMUST00000035056 ENSMUST00000062368 ENSMUST00000127744 ENSMUST00000111991 ENSMUST00000136969 ENSMUST00000165596 ENSMUST00000042561 ENSMUST00000108433 ENSMUST00000169547 ENSMUST00000028016 ENSMUST00000048957 ENSMUST00000079038 ENSMUST00000079812

Gene Information

Description Not Available

Gene Symbol:   Nkx3-2
Gene Name:   NK3 homeobox 2
Aliases:   Bapx1, NKX3.2, Nkx-3.2
RefSeq:   NC_000071.6 NT_039305.8
Ensembl:   ENSMUSG00000049691
Entrez:   12020
UniGene:   Mm.2530
Chromosome Mapping:   5 B3|5 23.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.680000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download