PrimePCR™ SYBR® Green Assay: Hoxc13, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020865
Assay Design:   Intron-spanning
Chromosome Location:   15:102921867-102927280question
Amplicon Length:   133
Splice Variants Targeted:   ENSMUST00000001700 ENSMUST00000124516 ENSMUST00000018637 ENSMUST00000075734 ENSMUST00000029759 ENSMUST00000023828 ENSMUST00000084563 ENSMUST00000049530 ENSMUST00000164614 ENSMUST00000111265 ENSMUST00000024849 ENSMUST00000023631 ENSMUST00000113829 ENSMUST00000099502 ENSMUST00000100074 ENSMUST00000159780 ENSMUST00000096197 ENSMUST00000025253 ENSMUST00000174805 ENSMUST00000044766 ENSMUST00000119422 ENSMUST00000114082 ENSMUST00000091252

Gene Information

Description Not Available

Gene Symbol:   Hoxc13
Gene Name:   Homeobox C13
Aliases:   Not Available
RefSeq:   NC_000081.6 NT_039621.8
Ensembl:   ENSMUSG00000001655
Entrez:   15422
UniGene:   Mm.207062
Chromosome Mapping:   15 F3|15 57.4 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.670000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download