PrimePCR™ SYBR® Green Assay: Ly6g6c, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Ly6g6c, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Ly6g6c, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020859
Assay Design:   Intron-spanning
Chromosome Location:   17:35065419-35067435question
Amplicon Length:   111
Splice Variants Targeted:   ENSMUST00000031750 ENSMUST00000086521 ENSMUST00000025520 ENSMUST00000056096 ENSMUST00000178372 ENSMUST00000069604 ENSMUST00000177289 ENSMUST00000110249 ENSMUST00000078357 ENSMUST00000036355 ENSMUST00000151224 ENSMUST00000113377 ENSMUST00000081670 ENSMUST00000100194 ENSMUST00000175941 ENSMUST00000056338 ENSMUST00000176114 ENSMUST00000176205 ENSMUST00000004728 ENSMUST00000036467 ENSMUST00000045896 ENSMUST00000112121 ENSMUST00000053271 ENSMUST00000106447 ENSMUST00000117859 ENSMUST00000064493 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Ly6g6c
Gene Name:   Lymphocyte antigen 6 complex, locus G6C
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000007044
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.950000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download