PrimePCR™ SYBR® Green Assay: Sfrp5, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020856
Assay Design:   Intron-spanning
Chromosome Location:   19:42199004-42201598question
Amplicon Length:   183
Splice Variants Targeted:   ENSMUST00000151311 ENSMUST00000150235 ENSMUST00000122958 ENSMUST00000154135 ENSMUST00000140332 ENSMUST00000154470 ENSMUST00000129435 ENSMUST00000165086 ENSMUST00000082141 ENSMUST00000025069 ENSMUST00000141889 ENSMUST00000107178 ENSMUST00000021335 ENSMUST00000076443 ENSMUST00000108499 ENSMUST00000100903 ENSMUST00000018966 ENSMUST00000049583 ENSMUST00000099766

Gene Information

Description Not Available

Gene Symbol:   Sfrp5
Gene Name:   Secreted frizzled-related sequence protein 5
Aliases:   AI605071, SARP3
RefSeq:   NC_000085.6 NT_039687.8
Ensembl:   ENSMUSG00000018822
Entrez:   54612
UniGene:   Mm.470071
Chromosome Mapping:   19|19 D1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.750000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download