PrimePCR™ SYBR® Green Assay: Tm4sf5, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Tm4sf5, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Tm4sf5, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020855
Assay Design:   Intron-spanning
Chromosome Location:   11:70505413-70510271question
Amplicon Length:   200
Splice Variants Targeted:   ENSMUST00000077115 ENSMUST00000167679 ENSMUST00000167002 ENSMUST00000053288 ENSMUST00000172818 ENSMUST00000123464 ENSMUST00000047721 ENSMUST00000040434 ENSMUST00000049343 ENSMUST00000085096 ENSMUST00000068411 ENSMUST00000105102 ENSMUST00000079413 ENSMUST00000114792 ENSMUST00000177939 ENSMUST00000100816 ENSMUST00000061350 ENSMUST00000100013 ENSMUST00000026699 ENSMUST00000090806 ENSMUST00000102688 ENSMUST00000064190 ENSMUST00000171108 ENSMUST00000163643 ENSMUST00000034281 ENSMUST00000108950 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Tm4sf5
Gene Name:   Transmembrane 4 superfamily member 5
Aliases:   2010003F10Rik, AI323987
RefSeq:   NC_000077.6 NT_096135.6 NT_187026.1
Ensembl:   ENSMUSG00000018919
Entrez:   75604
Chromosome Mapping:   11 B3|11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.520000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download