PrimePCR™ SYBR® Green Assay: Mrpl30, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020841
Assay Design:   Intron-spanning
Chromosome Location:   1:37892952-37895406question
Amplicon Length:   196
Splice Variants Targeted:   ENSMUST00000067784 ENSMUST00000105038 ENSMUST00000092883 ENSMUST00000106739 ENSMUST00000106741 ENSMUST00000033339 ENSMUST00000106440 ENSMUST00000177289 ENSMUST00000110249 ENSMUST00000094705 ENSMUST00000033160 ENSMUST00000015791 ENSMUST00000093906 ENSMUST00000033621 ENSMUST00000028119 ENSMUST00000027256 ENSMUST00000037913 ENSMUST00000107773 ENSMUST00000032469 ENSMUST00000078286 ENSMUST00000138502 ENSMUST00000036074 ENSMUST00000113753 ENSMUST00000105679 ENSMUST00000099525 ENSMUST00000152080 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Mrpl30
Gene Name:   Mitochondrial ribosomal protein L30
Aliases:   2310001L22Rik, MRP-L28, Rpml28
RefSeq:   NC_000067.6 NT_039170.8
Ensembl:   ENSMUSG00000026087
Entrez:   107734
UniGene:   Mm.26614
Chromosome Mapping:   1 B|1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.630000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download