PrimePCR™ SYBR® Green Assay: Batf3, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020840
Assay Design:   Intron-spanning
Chromosome Location:   1:191100784-191108604question
Amplicon Length:   173
Splice Variants Targeted:   ENSMUST00000027943 ENSMUST00000027667 ENSMUST00000163260 ENSMUST00000053464 ENSMUST00000067695 ENSMUST00000086763 ENSMUST00000004850 ENSMUST00000107363 ENSMUST00000107361 ENSMUST00000034452 ENSMUST00000122421 ENSMUST00000153273 ENSMUST00000040324 ENSMUST00000085222 ENSMUST00000107525 ENSMUST00000147005 ENSMUST00000166315 ENSMUST00000032192 ENSMUST00000097786 ENSMUST00000097785 ENSMUST00000115104

Gene Information

Description Not Available

Gene Symbol:   Batf3
Gene Name:   Basic leucine zipper transcription factor, ATF-like 3
Aliases:   9130211I03Rik, Snft
RefSeq:   NC_000067.6 NT_039189.8
Ensembl:   ENSMUSG00000026630
Entrez:   381319
UniGene:   Mm.6922
Chromosome Mapping:   1 H6|1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.610000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download