PrimePCR™ SYBR® Green Assay: Mtfp1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020832
Assay Design:   Intron-spanning
Chromosome Location:   11:4092270-4093884question
Amplicon Length:   84
Splice Variants Targeted:   ENSMUST00000066026 ENSMUST00000028970 ENSMUST00000027528 ENSMUST00000032627 ENSMUST00000004868 ENSMUST00000050555 ENSMUST00000022574 ENSMUST00000113200 ENSMUST00000028132 ENSMUST00000118022 ENSMUST00000067417 ENSMUST00000121312 ENSMUST00000159212 ENSMUST00000160260 ENSMUST00000111427 ENSMUST00000013773 ENSMUST00000174367 ENSMUST00000170329 ENSMUST00000031049 ENSMUST00000085733

Gene Information

Description Not Available

Gene Symbol:   Mtfp1
Gene Name:   Mitochondrial fission process 1
Aliases:   1700020C11Rik, 2610507A21Rik, AV005788, Mtp18
RefSeq:   NC_000077.6 NT_039515.7
Ensembl:   ENSMUSG00000004748
Entrez:   67900
UniGene:   Mm.41719
Chromosome Mapping:   11 A1|11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.840000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download