PrimePCR™ SYBR® Green Assay: Spry4, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020826
Assay Design:   Intron-spanning
Chromosome Location:   18:38590709-38601227question
Amplicon Length:   75
Splice Variants Targeted:   ENSMUST00000047309 ENSMUST00000025295 ENSMUST00000115336 ENSMUST00000044926 ENSMUST00000121010 ENSMUST00000174629 ENSMUST00000048112 ENSMUST00000057209 ENSMUST00000127638 ENSMUST00000043896 ENSMUST00000020209 ENSMUST00000018610 ENSMUST00000081125 ENSMUST00000108756 ENSMUST00000108757 ENSMUST00000129843 ENSMUST00000043141 ENSMUST00000161356 ENSMUST00000062372 ENSMUST00000036328 ENSMUST00000017783 ENSMUST00000097653 ENSMUST00000056925 ENSMUST00000046005 ENSMUST00000106738 ENSMUST00000094639 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Spry4
Gene Name:   Sprouty homolog 4 (Drosophila)
Aliases:   A030006O18Rik, sprouty4
RefSeq:   NC_000084.6 NT_039674.8
Ensembl:   ENSMUSG00000024427
Entrez:   24066
UniGene:   Mm.42038
Chromosome Mapping:   18 B3|18 18.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.910000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download