PrimePCR™ SYBR® Green Assay: 1700020L24Rik, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020818
Assay Design:   Intron-spanning
Chromosome Location:   11:83437711-83440345question
Amplicon Length:   76
Splice Variants Targeted:   ENSMUST00000048263 ENSMUST00000090027 ENSMUST00000151285 ENSMUST00000169910 ENSMUST00000059272 ENSMUST00000169266 ENSMUST00000163320 ENSMUST00000165239 ENSMUST00000005578 ENSMUST00000056665 ENSMUST00000025729 ENSMUST00000026315 ENSMUST00000009972 ENSMUST00000053748 ENSMUST00000092645 ENSMUST00000045633 ENSMUST00000114232 ENSMUST00000037378 ENSMUST00000102881 ENSMUST00000020321 ENSMUST00000045771 ENSMUST00000070577 ENSMUST00000030119 ENSMUST00000068125 ENSMUST00000069705 ENSMUST00000031057 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   1700020L24Rik
Gene Name:   RIKEN cDNA 1700020L24 gene
Aliases:   Not Available
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000035085
Entrez:   66330
UniGene:   Mm.44800
Chromosome Mapping:   11|11 B5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.180000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download