PrimePCR™ SYBR® Green Assay: Nxph4, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020805
Assay Design:   Intron-spanning
Chromosome Location:   10:127526908-127534418question
Amplicon Length:   69
Splice Variants Targeted:   ENSMUST00000006701 ENSMUST00000048112 ENSMUST00000057209 ENSMUST00000048560 ENSMUST00000172649 ENSMUST00000023965 ENSMUST00000112793 ENSMUST00000075591 ENSMUST00000078320 ENSMUST00000112790 ENSMUST00000112825 ENSMUST00000043881 ENSMUST00000019074 ENSMUST00000044687 ENSMUST00000026209 ENSMUST00000106746 ENSMUST00000075012 ENSMUST00000024894 ENSMUST00000095266 ENSMUST00000022728

Gene Information

Description Not Available

Gene Symbol:   Nxph4
Gene Name:   Neurexophilin 4
Aliases:   1110036M10Rik, AI851051
RefSeq:   NC_000076.6 NT_039500.8
Ensembl:   ENSMUSG00000040258
Entrez:   104080
UniGene:   Mm.437200
Chromosome Mapping:   10 D3|10

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.250000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download