PrimePCR™ SYBR® Green Assay: Wdr89, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: Wdr89, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Wdr89, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020792
Assay Design:   Intron-spanning
Chromosome Location:   12:75633434-75669531question
Amplicon Length:   105
Splice Variants Targeted:   ENSMUST00000093912 ENSMUST00000103033 ENSMUST00000125918 ENSMUST00000035548 ENSMUST00000047511 ENSMUST00000163821 ENSMUST00000111460 ENSMUST00000081790 ENSMUST00000166712 ENSMUST00000015791 ENSMUST00000061620 ENSMUST00000109896 ENSMUST00000173206 ENSMUST00000172288 ENSMUST00000140920 ENSMUST00000015511 ENSMUST00000020732 ENSMUST00000047441 ENSMUST00000081658 ENSMUST00000052872 ENSMUST00000071750 ENSMUST00000121227 ENSMUST00000120985 ENSMUST00000121932 ENSMUST00000120516 ENSMUST00000051810 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Wdr89
Gene Name:   WD repeat domain 89
Aliases:   2600001A11Rik
RefSeq:   NC_000078.6 NT_039551.8
Ensembl:   ENSMUSG00000045690
Entrez:   72338
Chromosome Mapping:   12 C3|12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 37.540000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download