PrimePCR™ SYBR® Green Assay: Fitm2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: Fitm2, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Fitm2, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020783
Assay Design:   Intron-spanning
Chromosome Location:   2:163470059-163472489question
Amplicon Length:   117
Splice Variants Targeted:   ENSMUST00000048478 ENSMUST00000052631 ENSMUST00000077191 ENSMUST00000098669 ENSMUST00000044547 ENSMUST00000033230 ENSMUST00000018333 ENSMUST00000145007 ENSMUST00000138439 ENSMUST00000130372 ENSMUST00000035148 ENSMUST00000020463 ENSMUST00000118498 ENSMUST00000109309 ENSMUST00000066545 ENSMUST00000039074 ENSMUST00000110052 ENSMUST00000096164 ENSMUST00000026209 ENSMUST00000063091 ENSMUST00000099967 ENSMUST00000126486 ENSMUST00000135242 ENSMUST00000146287 ENSMUST00000136659 ENSMUST00000032835 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Fitm2
Gene Name:   Fat storage-inducing transmembrane protein 2
Aliases:   AI414861, D930001I22Rik, Fit2
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000048486
Entrez:   228859
Chromosome Mapping:   2 H3|2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.180000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download