PrimePCR™ SYBR® Green Assay: Gm9949, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020774
Assay Design:   Intron-spanning
Chromosome Location:   18:62180569-62184034question
Amplicon Length:   118
Splice Variants Targeted:   ENSMUST00000001712 ENSMUST00000067743 ENSMUST00000028914 ENSMUST00000009727 ENSMUST00000033004 ENSMUST00000168189 ENSMUST00000009344 ENSMUST00000028951 ENSMUST00000094456 ENSMUST00000109875 ENSMUST00000109877 ENSMUST00000028250 ENSMUST00000080884 ENSMUST00000112713 ENSMUST00000030432 ENSMUST00000030439 ENSMUST00000094853 ENSMUST00000033509 ENSMUST00000041761 ENSMUST00000113725 ENSMUST00000115510 ENSMUST00000090632 ENSMUST00000115511 ENSMUST00000117556 ENSMUST00000169256 ENSMUST00000046349 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Gm9949
Gene Name:   Predicted gene 9949
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000054589
UniGene:   Mm.296917
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.270000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download