PrimePCR™ SYBR® Green Assay: Apol9b, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Apol9b, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Apol9b, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020773
Assay Design:   Intron-spanning
Chromosome Location:   15:77405652-77411080question
Amplicon Length:   80
Splice Variants Targeted:   ENSMUST00000061512 ENSMUST00000052164 ENSMUST00000025294 ENSMUST00000098154 ENSMUST00000098155 ENSMUST00000112027 ENSMUST00000150054 ENSMUST00000042601 ENSMUST00000036355 ENSMUST00000076333 ENSMUST00000110525 ENSMUST00000110524 ENSMUST00000028652 ENSMUST00000049400 ENSMUST00000146647 ENSMUST00000115537 ENSMUST00000081776 ENSMUST00000152564 ENSMUST00000146948 ENSMUST00000141740 ENSMUST00000138950 ENSMUST00000057272

Gene Information

Description Not Available

Gene Symbol:   Apol9b
Gene Name:   Apolipoprotein L 9b
Aliases:   Not Available
RefSeq:   NC_000081.6 NT_039621.8
Ensembl:   ENSMUSG00000057346
Entrez:   223672
Chromosome Mapping:   15 E1|15

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.410000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download