PrimePCR™ SYBR® Green Assay: Gm16314, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020757
Assay Design:   Intron-spanning
Chromosome Location:   16:18499073-18501021question
Amplicon Length:   120
Splice Variants Targeted:   ENSMUST00000035007 ENSMUST00000005431 ENSMUST00000129404 ENSMUST00000105426 ENSMUST00000131321 ENSMUST00000126920 ENSMUST00000147189 ENSMUST00000105424 ENSMUST00000092420 ENSMUST00000105429 ENSMUST00000131724 ENSMUST00000151116 ENSMUST00000124046 ENSMUST00000149977 ENSMUST00000177107 ENSMUST00000064562 ENSMUST00000027667 ENSMUST00000163260 ENSMUST00000132383 ENSMUST00000031091 ENSMUST00000111702 ENSMUST00000032383 ENSMUST00000111701 ENSMUST00000053273 ENSMUST00000079573 ENSMUST00000030865 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Gm16314
Gene Name:   Predicted gene 16314
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000086965
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.990800
y-intercept 33.140000
Efficiency 114

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download