PrimePCR™ SYBR® Green Assay: Gpr45, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020752
Assay Design:   Intron-spanning
Chromosome Location:   1:42953175-43032111question
Amplicon Length:   189
Splice Variants Targeted:   ENSMUST00000047875 ENSMUST00000106489 ENSMUST00000106487 ENSMUST00000106488 ENSMUST00000035661 ENSMUST00000179766 ENSMUST00000170629 ENSMUST00000063199 ENSMUST00000027886 ENSMUST00000172057 ENSMUST00000171292

Gene Information

Description Not Available

Gene Symbol:   Gpr45
Gene Name:   G protein-coupled receptor 45
Aliases:   9230112G11Rik, AW046772
RefSeq:   NC_000067.6 NT_039170.8
Ensembl:   ENSMUSG00000041907
Entrez:   93690
UniGene:   Mm.444976
Chromosome Mapping:   1|1 C1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.220000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download