PrimePCR™ SYBR® Green Assay: Olfr544, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020737
Assay Design:   Intron-spanning
Chromosome Location:   7:102485092-102488307question
Amplicon Length:   173
Splice Variants Targeted:   ENSMUST00000098789 ENSMUST00000089085 ENSMUST00000149440 ENSMUST00000027601 ENSMUST00000051201 ENSMUST00000084589 ENSMUST00000053413 ENSMUST00000166957 ENSMUST00000038336 ENSMUST00000061639 ENSMUST00000034863 ENSMUST00000160505 ENSMUST00000162443 ENSMUST00000034281 ENSMUST00000108951 ENSMUST00000108950 ENSMUST00000098796 ENSMUST00000115351 ENSMUST00000122211 ENSMUST00000093942

Gene Information

Olfactory receptors interact with odorant molecules in the nose to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq Jul 2008]

Gene Symbol:   Olfr544
Gene Name:   Olfactory receptor 544
Aliases:   MOR42-3, Ors6
RefSeq:   NC_000073.6 NT_039433.8
Ensembl:   ENSMUSG00000043925
Entrez:   257926
UniGene:   Mm.103733
Chromosome Mapping:   7 E3|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.510000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download