PrimePCR™ SYBR® Green Assay: Gm5622, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020715
Assay Design:   Intron-spanning
Chromosome Location:   14:51553019-51654894question
Amplicon Length:   188
Splice Variants Targeted:   ENSMUST00000001712 ENSMUST00000096170 ENSMUST00000092434 ENSMUST00000092432 ENSMUST00000092431 ENSMUST00000054167 ENSMUST00000114236 ENSMUST00000102611 ENSMUST00000018887 ENSMUST00000092984 ENSMUST00000112512 ENSMUST00000031477 ENSMUST00000025468 ENSMUST00000135688 ENSMUST00000037007 ENSMUST00000047511 ENSMUST00000163821 ENSMUST00000032915 ENSMUST00000086693 ENSMUST00000097291 ENSMUST00000177034 ENSMUST00000145322 ENSMUST00000081857 ENSMUST00000161178 ENSMUST00000050349 ENSMUST00000037055 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Gm5622
Gene Name:   Predicted gene 5622
Aliases:   EG434459
RefSeq:   NC_000080.6 NT_039606.8
Ensembl:   ENSMUSG00000079244
Entrez:   434459
UniGene:   Mm.359230
Chromosome Mapping:   14 C2|14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 34.690000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download