PrimePCR™ SYBR® Green Assay: Nacc1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020700
Assay Design:   Intron-spanning
Chromosome Location:   8:84677190-84687838question
Amplicon Length:   197
Splice Variants Targeted:   ENSMUST00000027690 ENSMUST00000041023 ENSMUST00000161934 ENSMUST00000001975 ENSMUST00000027517 ENSMUST00000022218 ENSMUST00000102743 ENSMUST00000101206 ENSMUST00000177955 ENSMUST00000033901 ENSMUST00000178721 ENSMUST00000087315 ENSMUST00000173584 ENSMUST00000173212 ENSMUST00000114292 ENSMUST00000120594 ENSMUST00000120898 ENSMUST00000056889 ENSMUST00000112392 ENSMUST00000112390 ENSMUST00000026975

Gene Information

Description Not Available

Gene Symbol:   Nacc1
Gene Name:   Nucleus accumbens associated 1, BEN and BTB (POZ) domain containing
Aliases:   2010001H03Rik, 4930511N13Rik, Btbd14b, Nac1
RefSeq:   NC_000074.6 NT_078575.7
Ensembl:   ENSMUSG00000001910
Entrez:   66830
UniGene:   Mm.289698
Chromosome Mapping:   8 C3|8 35.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 34.950000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download