PrimePCR™ SYBR® Green Assay: Cryba2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Cryba2, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Cryba2, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020698
Assay Design:   Intron-spanning
Chromosome Location:   1:74890088-74891977question
Amplicon Length:   194
Splice Variants Targeted:   ENSMUST00000058914 ENSMUST00000065162 ENSMUST00000166193 ENSMUST00000006587 ENSMUST00000094644 ENSMUST00000121085 ENSMUST00000119990 ENSMUST00000144700 ENSMUST00000078953 ENSMUST00000118317 ENSMUST00000106845 ENSMUST00000029438 ENSMUST00000161861 ENSMUST00000162671 ENSMUST00000069284 ENSMUST00000172181 ENSMUST00000161188 ENSMUST00000034326 ENSMUST00000033899 ENSMUST00000034429 ENSMUST00000035208 ENSMUST00000006721 ENSMUST00000021539 ENSMUST00000085026 ENSMUST00000072040 ENSMUST00000032172 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Cryba2
Gene Name:   Crystallin, beta A2
Aliases:   E130107M19Rik
RefSeq:   NC_000067.6 NT_039170.8
Ensembl:   ENSMUSG00000006546
Entrez:   12958
Chromosome Mapping:   1 C3|1 40.8 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.990000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download