PrimePCR™ SYBR® Green Assay: Uchl3, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $134.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020693
Assay Design:   Intron-spanning
Chromosome Location:   14:101654021-101665860question
Amplicon Length:   167
Splice Variants Targeted:   ENSMUST00000006444 ENSMUST00000039229 ENSMUST00000003876 ENSMUST00000115765 ENSMUST00000120267 ENSMUST00000122116 ENSMUST00000023061 ENSMUST00000064473 ENSMUST00000137551 ENSMUST00000069817 ENSMUST00000099981 ENSMUST00000134720 ENSMUST00000148747 ENSMUST00000111882 ENSMUST00000111846 ENSMUST00000034203 ENSMUST00000174398 ENSMUST00000111404 ENSMUST00000111254 ENSMUST00000020315 ENSMUST00000053734 ENSMUST00000039011 ENSMUST00000057610 ENSMUST00000113200 ENSMUST00000028132 ENSMUST00000078588 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Uchl3
Gene Name:   Ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
Aliases:   Not Available
RefSeq:   NC_000080.6 NT_039606.8
Ensembl:   ENSMUSG00000022111
Entrez:   50933
UniGene:   Mm.275970
Chromosome Mapping:   14 E2.3|14 48.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.390000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download