PrimePCR™ SYBR® Green Assay: Fam158a, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: Fam158a, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Fam158a, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020691
Assay Design:   Intron-spanning
Chromosome Location:   14:55581656-55584975question
Amplicon Length:   199
Splice Variants Targeted:   ENSMUST00000107862 ENSMUST00000055401 ENSMUST00000096904 ENSMUST00000022828 ENSMUST00000066997 ENSMUST00000143663 ENSMUST00000089394 ENSMUST00000119509 ENSMUST00000119943 ENSMUST00000121091 ENSMUST00000041683 ENSMUST00000043674 ENSMUST00000061318

Gene Information

Description Not Available

Gene Symbol:   Fam158a
Gene Name:   Family with sequence similarity 158, member A
Aliases:   1500005A01Rik, Cgi112, Fam158a
RefSeq:   NC_000080.6 NT_039606.8
Ensembl:   ENSMUSG00000022217
Entrez:   85308
Chromosome Mapping:   14 C3|14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.840000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download