PrimePCR™ SYBR® Green Assay: 2410022L05Rik, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020685
Assay Design:   Intron-spanning
Chromosome Location:   8:13885784-13887801question
Amplicon Length:   110
Splice Variants Targeted:   ENSMUST00000046071 ENSMUST00000149756 ENSMUST00000031651 ENSMUST00000055911 ENSMUST00000022881 ENSMUST00000110438 ENSMUST00000131035 ENSMUST00000093902 ENSMUST00000089059 ENSMUST00000079247 ENSMUST00000031547 ENSMUST00000129643 ENSMUST00000058240 ENSMUST00000153871 ENSMUST00000102491 ENSMUST00000097816 ENSMUST00000168157 ENSMUST00000040222 ENSMUST00000069958 ENSMUST00000057927 ENSMUST00000148532 ENSMUST00000032279 ENSMUST00000074067 ENSMUST00000108640 ENSMUST00000108639 ENSMUST00000032732 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   2410022L05Rik
Gene Name:   RIKEN cDNA 2410022L05 gene
Aliases:   1700029I03Rik, AA409325, AI256813, C85432, Copr5
RefSeq:   NC_000074.6 NT_039455.8
Ensembl:   ENSMUSG00000031458
Entrez:   66423
UniGene:   Mm.29063
Chromosome Mapping:   8 A1.1|8

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 34.820000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download