PrimePCR™ SYBR® Green Assay: Zbtb1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020675
Assay Design:   Intron-spanning
Chromosome Location:   12:76370566-76385290question
Amplicon Length:   200
Splice Variants Targeted:   ENSMUST00000079300 ENSMUST00000042701 ENSMUST00000036665 ENSMUST00000167107 ENSMUST00000034443 ENSMUST00000066882 ENSMUST00000119344 ENSMUST00000122210 ENSMUST00000055241 ENSMUST00000022051 ENSMUST00000118096 ENSMUST00000042850 ENSMUST00000035346 ENSMUST00000109784 ENSMUST00000037375 ENSMUST00000109650 ENSMUST00000046755 ENSMUST00000174874 ENSMUST00000109651 ENSMUST00000034267 ENSMUST00000072889 ENSMUST00000110398 ENSMUST00000037117 ENSMUST00000171404 ENSMUST00000115636 ENSMUST00000103115 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Zbtb1
Gene Name:   Zinc finger and BTB domain containing 1
Aliases:   9430077A12, AI256369, Bsg2, C430003J21Rik
RefSeq:   NC_000078.6 NT_039551.8
Ensembl:   ENSMUSG00000033454
Entrez:   268564
UniGene:   Mm.77065
Chromosome Mapping:   12 C3|12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.070000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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