PrimePCR™ SYBR® Green Assay: Spcs2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020671
Assay Design:   Intron-spanning
Chromosome Location:   7:99848976-99858880question
Amplicon Length:   171
Splice Variants Targeted:   ENSMUST00000105420 ENSMUST00000077925 ENSMUST00000046717 ENSMUST00000110774 ENSMUST00000110773 ENSMUST00000079934 ENSMUST00000136740 ENSMUST00000135298 ENSMUST00000128341 ENSMUST00000105650 ENSMUST00000055754 ENSMUST00000105651 ENSMUST00000140295 ENSMUST00000132064 ENSMUST00000124505 ENSMUST00000094447 ENSMUST00000042390 ENSMUST00000111254 ENSMUST00000029126 ENSMUST00000109685 ENSMUST00000057717 ENSMUST00000113585 ENSMUST00000089449 ENSMUST00000163271 ENSMUST00000036274

Gene Information

Description Not Available

Gene Symbol:   Spcs2
Gene Name:   Signal peptidase complex subunit 2 homolog (S. cerevisiae)
Aliases:   5730406I15Rik, AA408713, mKIAA0102
RefSeq:   NC_000073.6 NT_039433.8
Ensembl:   ENSMUSG00000035227
Entrez:   66624
UniGene:   Mm.30043
Chromosome Mapping:   7 E2|7

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.450000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download