This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qMmuCIP0032528
PrimePCR™ PreAmp for SYBR® Green Assay: Anapc2, Mouse
PrimePCR™ Template for SYBR® Green Assay: Anapc2, Mouse
This gene encodes a component of the anaphase promoting complex/cyclosome (APC/C) a cell cycle-regulated ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor for subsequent degradation by the proteasome. [provided by RefSeq Oct 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.