PrimePCR™ SYBR® Green Assay: Tle3, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0019269
Assay Design:   Intron-spanning
Chromosome Location:   9:61415605-61416664question
Amplicon Length:   60
Splice Variants Targeted:   ENSMUST00000159386 ENSMUST00000034820 ENSMUST00000162583 ENSMUST00000160882 ENSMUST00000162973 ENSMUST00000159050 ENSMUST00000161207 ENSMUST00000159630 ENSMUST00000161689 ENSMUST00000162127 ENSMUST00000178113

Gene Information

Description Not Available

Gene Symbol:   Tle3
Gene Name:   Transducin-like enhancer of split 3, homolog of Drosophila E(spl)
Aliases:   2610103N05Rik, ESG, Grg3a, Grg3b, mKIAA1547
RefSeq:   NC_000075.6 NT_039474.8
Ensembl:   ENSMUSG00000032280
Entrez:   21887
UniGene:   Mm.24255
Chromosome Mapping:   9 C|9

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 34.750000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download