PrimePCR™ SYBR® Green Assay: Cacna1b, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0019066
Assay Design:   Intron-spanning
Chromosome Location:   2:24761819-24762802question
Amplicon Length:   73
Splice Variants Targeted:   ENSMUST00000070864 ENSMUST00000102939 ENSMUST00000114447 ENSMUST00000041342 ENSMUST00000100348

Gene Information

Description Not Available

Gene Symbol:   Cacna1b
Gene Name:   Calcium channel, voltage-dependent, N type, alpha 1B subunit
Aliases:   AW050276, AW060892, AW822256, Cav2.2, Cchn1a, alpha(1B)
RefSeq:   NC_000068.7 NT_039206.8
Ensembl:   ENSMUSG00000004113
Entrez:   12287
UniGene:   Mm.4424
Chromosome Mapping:   2 A2|2 18.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.440000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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