This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: Klc1, Mouse
PrimePCR™ Template for SYBR® Green Assay: Klc1, Mouse
Conventional kinesin is a tetrameric molecule composed of two heavy chains and two light chains and transports various cargos along microtubules toward their plus ends. The heavy chains provide the motor activity while the light chains bind to various cargos. This gene encodes a member of the kinesin light chain family. It associates with kinesin heavy chain through an N-terminal domain and six tetratricopeptide repeat (TPR) motifs are thought to be involved in binding of cargos such as vesicles mitochondria and the Golgi complex. Thus kinesin light chains function as adapter molecules and not motors per se. Although previously named "kinesin 2" this gene is not a member of the kinesin-2 / kinesin heavy chain subfamily of kinesin motor proteins. Extensive alternative splicing produces isoforms with different C-termini that are proposed to bind to different cargos; however the full-length nature of some of these variants has not been determined. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.