PrimePCR™ SYBR® Green Assay: Triobp, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0033553

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Triobp, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Triobp, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0016532
Assay Design:   Intron-spanning
Chromosome Location:   15:78987892-78990879question
Amplicon Length:   90
Splice Variants Targeted:   ENSMUST00000109690 ENSMUST00000109689 ENSMUST00000109687 ENSMUST00000130663 ENSMUST00000144151 ENSMUST00000109688

Gene Information

This gene encodes a protein that interacts with trio which is involved with neural tissue development and controlling actin cytoskeleton organization cell motility and cell growth. The encoded protein also associates with F-actin and stabilizes F-actin structures. Domains contained in this encoded protein are an N-terminal pleckstrin homology domain and a C-terminal coiled-coil region. Mutations in the human gene have been associated with a form of autosomal recessive nonsyndromic deafness. Multiple alternatively spliced transcript variants have been described [provided by RefSeq Sep 2012]

Gene Symbol:   Triobp
Gene Name:   TRIO and F-actin binding protein
Aliases:   AI428493, EST478828, Tara, mKIAA1662
RefSeq:   NC_000081.6 NT_039621.8
Ensembl:   ENSMUSG00000033088
Entrez:   110253
Chromosome Mapping:   15 E1|15 46.6 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.400000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download