This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qMmuCIP0030028
PrimePCR™ PreAmp for SYBR® Green Assay: Aspa, Mouse
PrimePCR™ Template for SYBR® Green Assay: Aspa, Mouse
This gene encodes an enzyme that deacteylates N-acetyl-L-aspartic acid (NAA) in the brain to yield acetate and L-aspartate. In humans alterations in neuronal NAA concentration are associated with many neurodegenerative diseases (decrease associated with epilepsy multiple sclerosis myotrophic lateral sclerosis and Alzheimer's disease; increase associated with Canavan disease). In mouse mutations in this gene which cause accumulation of NAA result in demyelination and spongy degeneration in the CNS and serve as a pathophysiological model for Canavan disease. [provided by RefSeq Dec 2012]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.