This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qMmuCIP0030113
PrimePCR™ PreAmp for SYBR® Green Assay: Asxl2, Mouse
PrimePCR™ Template for SYBR® Green Assay: Asxl2, Mouse
This gene encodes a homolog of the Drosophila Asx gene which interacts with genes involved in axial patterning. Mice with mutations in this gene display abnormal patterning of the axial skeleton suggesting a similar function in mice as in Drosophila. This gene may also be involved in bone mineral density specifically osteoclastogenesis. [provided by RefSeq Aug 2012]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.