PrimePCR™ SYBR® Green Assay: Asxl2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0030113

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: Asxl2, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Asxl2, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0014674
Assay Design:   Intron-spanning
Chromosome Location:   12:3484498-3489675question
Amplicon Length:   120
Splice Variants Targeted:   ENSMUST00000092003 ENSMUST00000144247 ENSMUST00000153102 ENSMUST00000111215

Gene Information

This gene encodes a homolog of the Drosophila Asx gene which interacts with genes involved in axial patterning. Mice with mutations in this gene display abnormal patterning of the axial skeleton suggesting a similar function in mice as in Drosophila. This gene may also be involved in bone mineral density specifically osteoclastogenesis. [provided by RefSeq Aug 2012]

Gene Symbol:   Asxl2
Gene Name:   Additional sex combs like 2 (Drosophila)
Aliases:   4930556B16Rik, mKIAA1685
RefSeq:   NC_000078.6 NG_032909.1 NT_039548.8
Ensembl:   ENSMUSG00000037486
Entrez:   75302
Chromosome Mapping:   12 A1.1|12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 34.560000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download