PrimePCR™ SYBR® Green Assay: Adam22, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: Adam22, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Adam22, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0014560
Assay Design:   Intron-spanning
Chromosome Location:   5:8111961-8116617question
Amplicon Length:   68
Splice Variants Targeted:   ENSMUST00000088761 ENSMUST00000115386 ENSMUST00000050166 ENSMUST00000124121 ENSMUST00000154935 ENSMUST00000139048 ENSMUST00000139841 ENSMUST00000153427 ENSMUST00000136524 ENSMUST00000153889 ENSMUST00000123168 ENSMUST00000130315 ENSMUST00000136808 ENSMUST00000126384 ENSMUST00000046838 ENSMUST00000115388 ENSMUST00000088744

Gene Information

Description Not Available

Gene Symbol:   Adam22
Gene Name:   A disintegrin and metallopeptidase domain 22
Aliases:   2900022I03Rik, AI854032, MDC2
RefSeq:   NC_000071.6 NT_039299.7
Ensembl:   ENSMUSG00000040537
Entrez:   11496
Chromosome Mapping:   5 A1|5 0.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.850000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download