PrimePCR™ SYBR® Green Assay: Epb4.1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0014505
Assay Design:   Intron-spanning
Chromosome Location:   4:131978918-131982506question
Amplicon Length:   69
Splice Variants Targeted:   ENSMUST00000105970 ENSMUST00000105975 ENSMUST00000105974 ENSMUST00000054917 ENSMUST00000137846 ENSMUST00000105972 ENSMUST00000141291 ENSMUST00000030739 ENSMUST00000105981 ENSMUST00000084253

Gene Information

Description Not Available

Gene Symbol:   Epb4.1
Gene Name:   Erythrocyte protein band 4.1
Aliases:   4.1R, AI415518, D4Ertd442e, Elp-1, Elp1, Epb41, mKIAA4056
RefSeq:   NC_000070.6 NT_187033.1
Ensembl:   ENSMUSG00000028906
Entrez:   269587
UniGene:   Mm.30038
Chromosome Mapping:   4 D2.3|4 65.7 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.140000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download