PrimePCR™ SYBR® Green Assay: Phc3, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0031032

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0012103
Assay Design:   Intron-spanning
Chromosome Location:   3:30950927-30958047question
Amplicon Length:   114
Splice Variants Targeted:   ENSMUST00000168645 ENSMUST00000108255 ENSMUST00000099163 ENSMUST00000064718 ENSMUST00000177992 ENSMUST00000129817

Gene Information

Description Not Available

Gene Symbol:   Phc3
Gene Name:   Polyhomeotic-like 3 (Drosophila)
Aliases:   E030046K01Rik, Edr3, Hph3
RefSeq:   NC_000069.6 NT_162143.4
Ensembl:   ENSMUSG00000037652
Entrez:   241915
UniGene:   Mm.233173
Chromosome Mapping:   3 A3|3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 33.860000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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