PrimePCR™ SYBR® Green Assay: Ankrd26, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0032167

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0009857
Assay Design:   Intron-spanning
Chromosome Location:   6:118559349-118561893question
Amplicon Length:   88
Splice Variants Targeted:   ENSMUST00000112830

Gene Information

Description Not Available

Gene Symbol:   Ankrd26
Gene Name:   Ankyrin repeat domain 26
Aliases:   5730521P14Rik, BC008111, mKIAA1074
RefSeq:   NC_000072.6 NT_039353.8
Ensembl:   ENSMUSG00000007827
Entrez:   232339
UniGene:   Mm.276116
Chromosome Mapping:   6 F1|6

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 33.880000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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